Bromophenol Blue

Bromophenol Blue



From: £124.52

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Buy Bromophenol Blue from ReAgent UK

With over 40 years of experience, ReAgent is internationally recognised for its high-quality products and industry-leading customer service.

ReAgent supply powdered bromophenol blue in 5 kg and 25 kg containers. We can also supply ready-made 0.04% bromophenol solution in 500 ml and 2.5 L containers. Buy from our online chemicals store where you can get discounts on bulk orders and nationwide next-day delivery.


Bromophenol blue can be used as a pH indicator, a dye and a colour marker. It’s usually prepared by gently mixing bromine with phenolsulfonphthalein in glacial acetic acid.

  • pH indicator: it changes colour from yellow at pH 3 to purple at pH 4.6 in a reversible reaction. In chemical terms, the reaction is akin to what happens to phenolphthalein, another popular pH indicator.
  • Colour marker: it is popularly used to monitor progress during electrophoresis (both agarose gel and polyacrylamide gel). As this compound has a slightly negative charge, it will travel in the gel closely to the DNA or protein being tested. For example, using a 1% agarose gel in TAE or TBE buffer, bromophenol blue travels at the same speed as a 500 base pair DNA fragment.
  • Dye: At pH 7 and above, this compound absorbs red light and transmits blue light, which means solutions are blue. In acidic solutions (pH<7), this dye absorbs ultraviolet light and transmits in the yellow range. Interestingly, at pH 3.6 (when the colour changes), this compound has a green or red colour depending on the concentration. This is called dichromatic colour.

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All of our products are backed by a 100% quality guarantee so that you can buy with confidence. All of our procedures are regularly audited to ensure that we comply with stringent standards of quality.

We have a long list of chemicals available, including bespoke products, so that your needs are covered. If you need any further information, simply contact us and a member of our customer service team will be happy to help.




MSDS Files

The following files are available to download:

Product IDProductpost_statusBulkDownload SDS File

Please note: we only deliver to registered UK businesses at non-residential addresses with a minimum order value of £100 before VAT

We only supply to mainland UK, contact us on 0800 990 3258 for rates to Ireland.

Please notify us if you have specific delivery requirements such as restricted access, requirement for a tail-lift delivery vehicle or pallet truck. Failure to notify us of a special delivery instructions can incur additional charges from our carriers.

0800 990 3258